Diagnostic Tests for Syphilis

Demonstration of Treponema pallidum Using Dark-Field Microscopy

Demonstration of Treponema pallidum Using Dark-Field Microscopy

Dark field microscopy is the most specific and sensitive technique to diagnose syphilis when an active chancre or condyloma lata is present.

TPHA: Principle, Procedure, Results, Interpretations

TPHA: Principle, Procedure, Results, Interpretations

TPHA is a treponemal test for the serologic diagnosis of syphilis, based on the principle of passive hemagglutination. It detects IgG and IgM.

RPR Test: Principle, Procedure, Interpretations

RPR Test: Principle, Procedure, Interpretations

RPR test is a macroscopic, nontreponemal flocculation card test used to screen for syphilis. RPR test measures IgM and IgG antibodies to lipoidal material.

Laboratory diagnosis of Syphilis

Laboratory diagnosis of Syphilis

Syphilis is diagnosed by using dark field microscopy, non-treponemal tests (VDRL, RPR) or treponemal tests (TPHA, FTA-ABS).

FTA-ABS Test: Principle, Procedure, Results

FTA-ABS Test: Principle, Procedure, Results

FTA-ABS is an indirect fluorescent antibody technique used as a confirmatory test for syphilis. FTA-ABS is the first serological test to become positive following infection.

VDRL Test: Principle, Procedure, Results

VDRL Test: Principle, Procedure, Results

Venereal disease research laboratory (VDRL) test is a nonspecific flocculation test for the screening of syphilis.