Tinsdale agar base medium was developed by Tinsdale for the selective isolation and differentiation of C. diphtheriae from diphtheroids. Billings later modified the medium improving its differential qualities and reproducibility. Moore and Parsons used Billings’ […]
Culture media play a pivotal role in any microbiology laboratory for isolation, identification and sensitivity testing of different pathogens. Most of the laboratories usually prepare their own media or purchase prepared media for routine diagnostics […]
Brain Heart Infusion (BHI) broth is a general-purpose liquid medium used for the cultivation and maintenance of a wide variety of fastidious and non-fastidious microorganisms, including aerobic and anaerobic bacteria, yeast and molds from a […]
Kligler’s Iron Agar (KIA) is used for the detection of carbohydrate fermentation. Reactions of KIA helps to include/exclude particular bacterial isolate in the family Enterobacteriaceae. If an organism cannot ferment glucose, then in KIA, alkaline […]
Staib medium also known as Bird Seed Agar is a selective and differential medium for isolation of Cryptococcus neoformans from clinical specimens and differentiation of it from other Cryptococcus species. It is also known as […]
Chromogenic Culture Media are culture medium used to isolate, identify, and differentiate specific microorganism from a heterogeneous population. The medium contains chromogenic substrate which is utilized by the microorganism to give colored colonies that is specific […]
Cary-Blair transport medium can be used to transport of clinical specimens suspected to contain enteric pathogens, including Shigella, Salmonella, Vibrio cholerae, and Escherichia coli O157:H7. Since this transport medium has a high pH (8.4), viability […]
Alkaline peptone water (APW) is recommended as an enrichment broth for isolating Vibrio cholerae from clinical samples and non clinical samples (suspected food & water samples). A number of other broth media have been described for enrichment of […]
Nutrient Agar is used as a general-purpose medium for the growth of a wide variety of non-fastidious microorganisms. It consists of peptone, beef extract, and agar. This relatively simple formulation provides the nutrients necessary for the replication of a […]
The NNN (Novy-MCNeal-Nicolle), medium is a common and world known medium for isolation of the agent of the genus Leishmania, that causes Leishmaniasis (cutaneous, mucocutaneous or visceral) . Culture of this organism in a suitable media give rise […]
New York City (NYC) Medium is primarily designed for isolation of pathogenic Neisseria. It also supports the growth of genital mycoplasmas (Mycoplasma hominis and Ureaplasma urealyticum). New York City (NYC) medium is useful in the diagnosis […]
Chocolate Agar (CAP) is the lysed blood agar. The name is itself derived from the fact that red blood cell (RBC) lysis gives the medium a chocolate-brown color. Chocolate agar is used for the isolation […]
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