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HomeAntigen-Antibody Reactions

Antigen-Antibody Reactions

Principle of radioimmunoassay
Immunology

Radioimmunoassay: Principle, Uses, and limitations

June 11, 2020 Acharya Tankeshwar 0

Radioimmunoassay (RIA) is very sensitive (can detect at a concentration of <0.01 μg/mL) and a specific technique that is used for the quantitative detection of antigens or haptens. RIA is an extremely important tool in […]

Toxin neutralization test
Immunology

Neutralization Test for Virus and Toxins

June 9, 2020 Acharya Tankeshwar 0

When a person is infected with a pathogen, antibodies are produced against many epitopes of this pathogen. A subset of these antibodies can block infection by a process called neutralization. These antibodies block or distort the […]

Fluorescent antibody methods
Immunology

Indirect Fluorescent Antibody (IFA) Test

June 9, 2020 Acharya Tankeshwar 0

The indirect fluorescent antibody test (IFA) is a semi-quantitative, sensitive, and rapid test to detect which can be used to detect specific antibodies or antigens present in the samples. IFA for the detection of Antibodies […]

Immunology

Antigens in disease diagnosis

October 22, 2019 Acharya Tankeshwar 0

Detection of specific antigens present in the sample (blood, urine, CSF or stool) using immunologic methods such as precipitin tests, particle agglutination tests, immunofluorescence, immunochromatographic or enzyme immunoassays are the mainstay for the rapid diagnosis […]

Immunology

Antibodies in Disease Diagnosis

February 1, 2019 Acharya Tankeshwar 2

Antibodies are glycoproteins produced by our body in response to invading organisms or toxins (antigens). When a pathogen (bacteria, virus, protozoa or helminth) enters our body and anyhow manages to reach our blood stream, antigen […]

Immunology

Direct ELISA (Sandwich ELISA) Test for Antigen Detection

April 11, 2012 Acharya Tankeshwar 2

Enzyme-linked immunosorbent assay (ELISA) is extremely sensitive test that is used to detect specific antibodies or antigens. The direct ELISA is a test for the detection of antigen using specific immobilized antibody. In this procedure, […]

Immunology

ELISA Test: Principle, Materials, Procedure and Results

April 10, 2012 Acharya Tankeshwar 6

Enzyme-linked immunosorbent assay (ELISA) test is the most widely used type of immunoassay. ELISA is a rapid test used for detecting or quantifying antibody (Ab) against viruses, bacteria and other materials or antigen (Ag). ELISA  is so named […]

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