Antibiotic Susceptibility testing Archives

Antibiotic Resistance: Origin, Causes, Mechanism and Prevention

May 30, 2017 Srijana Khanal 2

This post was most recently updated on October 13th, 2019Antibiotics are the main therapeutic tools to treat various bacterial infections. But today, more and more antibiotics are becoming less effective. It is because of the […]

Lists of Bacterial Pathogens Associated with Intrinsic Antibiotic Resistance

May 14, 2017 tankeshwar 2

This post was most recently updated on March 26th, 2019Generally, when people (either general public or a physician or laboratory personnel) listen/read the term “antibiotic resistance” they think that, a bacterium which was previously sensitive […]

List of antibiotic-resistant “priority pathogens” according to WHO

February 27, 2017 tankeshwar 4

This post was most recently updated on March 26th, 2019World Health Organization (WHO) today published its first ever list of antibiotic-resistant “priority pathogens” – a catalogue of 12 families of bacteria that pose the greatest […]

Preparation of McFarland Turbidity Standards

June 9, 2016 tankeshwar 15

This post was most recently updated on May 13th, 2017McFarland turbidity standards are prepared by mixing various volumes of 1% sulfuric acid and 1% barium chloride to obtain solutions with specific optical densities. 0.5 McFarland […]

Nitrocefin test: Principle, procedure, uses and limitations

April 23, 2015 tankeshwar 3

This post was most recently updated on May 25th, 2015The nitrocefin biochemical test is a sensitive technique for detecting beta-lactamase producing strains of N. gonorrhoeae, H. influenzae, Staphylococcus spp, Enterococcus spp and Moraxella (Branhamella) catarrhalis. Nitrocefin […]

E-TEST ( Epsilometer test): Principle, purpose, procedure, results and interpretations

January 9, 2015 tankeshwar 6

This post was most recently updated on May 13th, 2017Epsilometer test (E- test ) is an ‘exponential gradient’ method of determination of antimicrobial resistance. The E-test has been developed to provide a direct quantification of antimicrobial […]

Modified Hodge Test (MHT) for Carbapenemase detection: Principle, Method and Interpretations

January 6, 2015 tankeshwar 1

This post was most recently updated on May 13th, 2017Modified Hodge Test (MHT) is a simple phenotypic test for detection of presence of carbapenemase enzyme in bacteria. Positive MHT test is observed in Klebsiella pneumoniae […]

Stokes Disc Diffusion method: Principle, Procedure and Interpretation of results

December 28, 2014 tankeshwar 4

This post was most recently updated on August 26th, 2019Stokes Disc Diffusion Technique varies from Kirby Bauer disc diffusion in the use of both control and test strain on a same plate. Stokes disc diffusion technique […]

How to store commercial antimicrobial discs

August 26, 2013 tankeshwar 1

This post was most recently updated on June 9th, 2016Cartridges containing commercially prepared antimicrobial discs specifically for susceptibility testing are generally packaged to ensure appropriate anhydrous conditions. Discs should be stored as follows: Refrigerate the containers […]

Mueller Hinton Agar (MHA): Composition, preparation and uses

July 20, 2013 tankeshwar 10

This post was most recently updated on September 9th, 2019Mueller-Hinton agar (MHA) is the best medium to use for routine antimicrobial susceptibility testing using Kirby-Bauer disc diffusion method for nonfastidious bacteria (both aerobe and facultative anaerobe). Use of […]

Guidelines for Routine Antibiotic Susceptibility testing (AST)

July 20, 2013 tankeshwar 2

This post was most recently updated on May 13th, 2017Always remember: Antibiotic Susceptibility testing should be performed on clinically significant isolates, not on all microorganisms recovered in culture. World Health Organization (WHO) recommends routine use of […]