Antibiotics are the main therapeutic tools to treat various bacterial infections. But today, more and more antibiotics are becoming less effective. It is because of the antibiotic resistance developed by bacteria due to the use […]
This post was most recently updated on March 26th, 2019Generally, when people (either general public or a physician or laboratory personnel) listen/read the term “antibiotic resistance” they think that, a bacterium which was previously sensitive […]
This post was most recently updated on March 26th, 2019World Health Organization (WHO) today published its first ever list of antibiotic-resistant “priority pathogens” – a catalogue of 12 families of bacteria that pose the greatest […]
This post was most recently updated on May 13th, 2017McFarland turbidity standards are prepared by mixing various volumes of 1% sulfuric acid and 1% barium chloride to obtain solutions with specific optical densities. 0.5 McFarland […]
This post was most recently updated on May 25th, 2015The nitrocefin biochemical test is a sensitive technique for detecting beta-lactamase producing strains of N. gonorrhoeae, H. influenzae, Staphylococcus spp, Enterococcus spp and Moraxella (Branhamella) catarrhalis. Nitrocefin […]
This post was most recently updated on May 13th, 2017Epsilometer test (E- test ) is an ‘exponential gradient’ method of determination of antimicrobial resistance. The E-test has been developed to provide a direct quantification of antimicrobial […]
This post was most recently updated on May 13th, 2017Modified Hodge Test (MHT) is a simple phenotypic test for detection of presence of carbapenemase enzyme in bacteria. Positive MHT test is observed in Klebsiella pneumoniae […]
This post was most recently updated on May 13th, 2017Stokes Disc Diffusion Technique varies from Kirby Bauer disc diffusion in the use of both control and test strain on a same plate. Stokes disc diffusion technique […]
This post was most recently updated on June 9th, 2016Cartridges containing commercially prepared antimicrobial discs specifically for susceptibility testing are generally packaged to ensure appropriate anhydrous conditions. Discs should be stored as follows: Refrigerate the containers […]
This post was most recently updated on June 9th, 2016Mueller-Hinton agar (MHA) is the best medium to use for routine susceptibility testing using Kirby-Bauer disc diffusion method for nonfastidious bacteria (both aerobe and facultative anaerobe). Use of media other […]
This post was most recently updated on May 13th, 2017Always remember: Antibiotic Susceptibility testing should be performed on clinically significant isolates, not on all microorganisms recovered in culture. World Health Organization (WHO) recommends routine use of […]
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