Anaerobiosis

GasPak Anaerobic System: Principle, Application

GasPak Anaerobic System: Principle, Application

Simple to use, best for laboratories with less sample load and the most commonly used method for anaerobiosis.

Anaerobic infections: Etiology, Characteristics, Diagnosis

Anaerobic infections: Etiology, Characteristics, Diagnosis

Anaerobic bacteria are part of the normal flora but can cause abscess formation and tissue necrosis when enter into sterile sites.

Robertson’s Cooked Meat (RCM) Medium

Robertson’s Cooked Meat (RCM) Medium

The most widely used anaerobic culture medium containing chopped meat particles and used for maintenance of stock cultures.

Thioglycollate broth: Composition, Principle, and Uses

Thioglycollate broth: Composition, Principle, and Uses

Thioglycollate broth supports the growth of anaerobes, aerobes, microaerophilic, and fastidious microorganisms.

McIntosh and Fildes’ Anaerobic Jar

McIntosh and Fildes’ Anaerobic Jar

McIntosh and Fildes' anaerobic jar works on the principle of evacuation and replacement, where the air inside the chamber is evacuated and replaced with mixture of gases 

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Clinical Specimens for Anaerobic Culture

Sample from sites known to have anaerobes as part of the normal flora (eg mouth) is unacceptable for anaerobic culture.

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Importance of Gram Stain in Anaerobic Bacteriology

Positive gram stain with negative culture report gives information regarding the adequacy of sample collection, transport, and also of culture methods used.

Anaerobic Culture Media

Anaerobic Culture Media

Commonly used anaerobic culture media are; Robertson's cooked meat broth, anaerobic blood agar, egg-yolk agar, bacteroides bile esculin agar.

Cultivation of Aerobic and Anaerobic Bacteria

Cultivation of Aerobic and Anaerobic Bacteria