Simple to use, best for laboratories with less sample load and the most commonly used method for anaerobiosis.
Anaerobic bacteria are part of the normal flora but can cause abscess formation and tissue necrosis when enter into sterile sites.
The most widely used anaerobic culture medium containing chopped meat particles and used for maintenance of stock cultures.
Thioglycollate broth supports the growth of anaerobes, aerobes, microaerophilic, and fastidious microorganisms.
McIntosh and Fildes' anaerobic jar works on the principle of evacuation and replacement, where the air inside the chamber is evacuated and replaced with mixture of gases
Sample from sites known to have anaerobes as part of the normal flora (eg mouth) is unacceptable for anaerobic culture.
Positive gram stain with negative culture report gives information regarding the adequacy of sample collection, transport, and also of culture methods used.
Commonly used anaerobic culture media are; Robertson's cooked meat broth, anaerobic blood agar, egg-yolk agar, bacteroides bile esculin agar.
