How to recover bacterial isolates from long term storage

This post was most recently updated on June 9th, 2016

This blog post contains general information regarding the recovery of a bacterial isolate from a frozen or lyophilized storage condition.

To recover the isolate follow the step wise procedure as mentioned below;

  1. Remove the frozen cultures from the freezer and place them on dry ice or into an alcohol and dry-ice bath;
  2.  Transfer to a laboratory safety cabinet or a clean area if a cabinet is not available.
  3. Scrape the top-most portion of the culture using a sterile loop
  4. Transfer to a growth medium without contaminating the top or inside of the vial.
  5. Re-close the vial before the contents completely thaw, and return the vial to the freezer; with careful technique, transfers can be successfully made from the same vial several times.
  6. Incubate 18–24 hours at 35–37°C; perform at least one subculture before using the isolate to inoculate a test.
About Acharya Tankeshwar 401 Articles
Hello, thank you for visiting my blog. I am Tankeshwar Acharya. Blogging is my passion. I am working as an Asst. Professor and Microbiologist at Department of Microbiology and Immunology, Patan Academy of Health Sciences, Nepal. If you want me to write about any posts that you found confusing/difficult, please mention in the comments below.

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