Minimum requirements for definitive identification of Pseudomonas aeruginosa

If a Microbiologist isolated a pathogenic bacteria in the laboratory and suspected that this pathogenic bacteria is a Pseudomonas aeruginosa based on its Gram staining characteristics, colony morphology and biochemical tests.

Before reporting the isolate as Pseudomonas aeruginosa, the isolate must have fulfilled the following minimum requirements.

  1. Gram staining: Gram negative rod
  2. Oxidase positive
  3. Typical smell (fruity grape-like odor or corn tortilla)
  4. Recognizable colony morphology
    1. On blood agar or chocolate agar appear as give large colonies with metallic sheen, mucoid, rough, or pigmented (pyocyanin) and often Beta Hemolytic
    2. On MacConkey Agar colonies appear as lactose-negative with green pigmentation, or metallic sheen
About Acharya Tankeshwar 458 Articles
Hello, thank you for visiting my blog. I am Tankeshwar Acharya. Blogging is my passion. I am working as an Asst. Professor and Microbiologist at Department of Microbiology and Immunology, Patan Academy of Health Sciences, Nepal. If you want me to write about any posts that you found confusing/difficult, please mention in the comments below.

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