Microbiology Specimen Collection Guidelines

By Acharya Tankeshwar •  Updated: 05/04/22 •  2 min read

Proper collection and transport of the biological specimens to the laboratory for culture is the most important step in the recovery of pathogenic organisms responsible for infectious diseases. A poorly collected specimen may lead to failure to recover the causative organism(s) and/or result in the recovery of contaminating organisms. This will lead to incorrect or even harmful therapy if treatment is directed toward commensal or contaminant organisms.

The following are fundamentals to be considered when collecting microbiology specimens for culture:

  1. Collect the specimen from the actual site of infection, avoiding contamination from adjacent tissues, organs, or secretions
  2. Collect the specimen at optimal times (for example, early morning sputum for the culture of acid-fast bacillus) to provide the best chance of recovering causative microorganisms.
  3. Whenever possible, collect specimens prior to administration of antimicrobial agents.
  4. A sufficient quantity of material must be obtained to perform the test.
  5. Properly label the specimen and complete the test request form. Mention the specific source of the specimen.  Each culture container must have a legible label, with the following minimum information:
    1. Patient name
    2. Patient identification number
    3. Source of specimen
    4. Name of clinician
    5. Date/hour of collection
  6. To ensure optimal recovery of microorganisms:
    1. Use appropriate collection devices: sterile, leak-proof specimen containers.
    2. Use appropriate transport media (anaerobe transport vials, Cary-Blair media, M4RT for viral and Chlamydia cultures, and urine boric acid transport).
    3. Use appropriate culture media
    4. Minimize transport time. Maintain an appropriate environment between a collection of specimens and delivery to the laboratory.
  7. For orders with more than one test, ensure that the proper transport system is utilized. (For example, anaerobic culture requests need to be submitted in anaerobic transport media; bacteriology requests should not be in viral transport media; AFB requests should not be in anaerobic transport media.)

Acharya Tankeshwar

Hello, thank you for visiting my blog. I am Tankeshwar Acharya. Blogging is my passion. As an asst. professor, I am teaching microbiology and immunology to medical and nursing students at PAHS, Nepal. I have been working as a microbiologist at Patan hospital for more than 10 years.

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