CT Values
Lab Diagnosis of Viral Disease

Ct Value in SARS-CoV-2 RT-PCR

Last updated on June 14th, 2021The cycle threshold (Ct) can be defined as the thermal cycle number needed to amplify viral RNA at which the fluorescent signal exceeds that of the background and thus passes the […]

Embroyonated Egg culture for Viruses
Lab Diagnosis of Viral Disease

Embryonated Egg Cultures for Viruses

Last updated on May 22nd, 2021Embryonated chicken eggs are used for the cultivation of some viruses. The viruses grow in the cells of the embryo and membranes. Specimens are inoculated into pathogen-free fertilized eggs of […]

Viral Transport Medium (VTM)
Lab Diagnosis of Viral Disease

Viral Transport Media (VTM): Preparation, Uses

Last updated on May 30th, 2021Viral Transport Media (VTM) are suitable for collection, transport, maintenance and long-term freeze storage of clinical specimens containing viruses, chlamydia, mycoplasma or ureaplasma organisms. It maintains organism viability for 48 […]

Immunology

Antigens in disease diagnosis

Last updated on May 25th, 2021Detection of specific antigens present in the sample (blood, urine, CSF, or stool) using immunologic methods such as precipitin tests, particle agglutination tests, immunofluorescence, immunochromatographic or enzyme immunoassays are the […]

Heterophile-antibody testing
Lab Diagnosis of Viral Disease

Monospot test: Principle, Procedure, Uses

Last updated on June 2nd, 2021Monospot test is a form of the heterophile antibody test which is used for the rapid screening of infectious mononucleosis (mono).  Epstein–Barr virus (EBV) causes the disease infectious mononucleosis. Blood, […]