Filtration is the preferred method of sterilizing heat sensitive liquid and gases without exposure to denaturing heat. Rather than destroying contaminating microorganisms, it simply removes them. It is the method of choice for sterilizing antibiotic […]
Disinfection is a process whereby pathogenic organisms, but not necessarily all microorganisms or spores, are destroyed. Disinfection may be accomplished by physical or chemical means. A number of factors influence the activity of disinfectants such […]
The autoclave is a sealed device (similar to a pressure cooker) that kills microorganisms using saturated steam under pressure. The use of moist heat facilitates the killing of all microorganisms, including heat-resistant endospores which is […]
Ethylene oxide (ETO) has been widely used as a low-temperature sterilant. ETO is a colorless gas that is flammable and explosive but it is liquid at temperatures below 10.8oC. ETO is an effective sterilizing agent for heat- […]
Of all the methods available for sterilization (killing or removal of all microorganisms, including bacterial spores), moist heat in the form of saturated steam under pressure is the most widely used and the most dependable […]
Dry heat sterilization (killing or removal of all microorganisms, including bacterial spores) technique requires longer exposure time (1.5 to 3 hours) and higher temperatures than moist heat sterilization. Various available methods of dry heat sterilization are; hot […]
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