MALDI-TOF mass spectrometry is a versatile analytical technique to detect and characterize mixtures of organic molecules. In Microbiology, it is being used as a rapid, accurate and cost-effective method for the identification of microorganism (bacteria, […]
Deoxycholate Citrate Agar (DCA) is a selective and differential medium, recommended for the isolation of enteric pathogens particularly Salmonella and Shigella species. Deoxycholate Citrate Agar is a modification of Deoxycholate Agar formulated by Leifson. DCA […]
Bismuth Sulfite Agar (BS) is a selective as well as differential medium for isolation and presumptive identification of Salmonella spp especially Salmonella Typhi. Salmonella spp are the causative agent of various diseases like gastroenteritis, sepsis, enteric […]
Blood culture is one of the most simple and commonly used investigation to establish the etiology of bloodstream infections (i.e. detection of bacteremia, sepsis and fungemia). Early diagnosis and accurate identification of the bacteria or […]
Chromogenic Agar Media are culture medium used to isolate, identify, and differentiate specific microorganism from a heterogeneous population. The medium contains chromogenic substrate which is utilized by the microorganism to give colored colonies that is […]
Tryptic Soy Agar is a universal medium which supports the growth of wide variety of microorganisms (both gram positive as well as gram negative bacteria grow in this medium). It is used for the isolation […]
The target readers of this blog post are general people (people who do not have health science background) who want to get basic information about “Blood Culture” as they or their relatives have recently been […]
Streptococcus pneumoniae is one of the most common causes of community-acquired pneumonia (CAP). Pneumococcal pneumonia has a mortality rate of > 30%, depending on bacteremia, age and underlying diseases. When not properly diagnosed and treated, S. […]
Capsule stain is a type of differential stain which uses acidic and basic dyes to stain background & bacterial cells respectively so that presence of capsule is easily visualized. Capsule is synthesized in the cytoplasm […]
Thioglycollate broth is the enrichment broth most frequently used in diagnostic bacteriology. This broth supports growth of anaerobes, aerobes, microaerophilic, and fastidious microorganisms. It contains many nutritive factors, including casein, yeast, beef extracts, and vitamins […]
Urea breath test (UBT) is a qualitative and non-invasive rapid test for the diagnosis of Helicobacter pylori (the major cause of gastric and duodenal ulcers) infections. Helicobacter pylori is a fastidious bacterium which is known to be a potent producer of […]
Cary-Blair transport medium can be used to transport of clinical specimens suspected to contain enteric pathogens, including Shigella, Salmonella, Vibrio cholerae, and Escherichia coli O157:H7. Since this transport medium has a high pH (8.4), viability […]
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