Tryptic Soy Agar is a universal medium which supports the growth of wide variety of microorganisms (both gram positive as well as gram negative bacteria grow in this medium). It is used for the isolation […]
The target readers of this blog post are general people (people who do not have health science background) who want to get basic information about “Blood Culture” as they or their relatives have recently been […]
Streptococcus pneumoniae is one of the most common causes of community-acquired pneumonia (CAP). Pneumococcal pneumonia has a mortality rate of > 30%, depending on bacteremia, age and underlying diseases. When not properly diagnosed and treated, S. […]
Capsule stain is a type of differential stain which uses acidic and basic dyes to stain background & bacterial cells respectively so that presence of capsule is easily visualized. Capsule is synthesized in the cytoplasm […]
Thioglycollate broth is the enrichment broth most frequently used in diagnostic bacteriology. This broth supports growth of anaerobes, aerobes, microaerophilic, and fastidious microorganisms. It contains many nutrient factors, including casein, yeast, and beef extracts, and […]
Urea breath test (UBT) is a qualitative and non-invasive rapid test for the diagnosis of Helicobacter pylori (the major cause of gastric and duodenal ulcers) infections. Helicobacter pylori is a fastidious bacterium which is known to be a potent producer of […]
Cary-Blair transport medium can be used to transport of clinical specimens suspected to contain enteric pathogens, including Shigella, Salmonella, Vibrio cholerae, and Escherichia coli O157:H7. Since this transport medium has a high pH (8.4), viability […]
Alkaline peptone water (APW) is recommended as an enrichment broth for isolating Vibrio cholerae from clinical samples and non clinical samples (suspected food & water samples). A number of other broth media have been described for enrichment of […]
Salmonella-Shigella (SS) agar is a selective and differential medium . It is used for the isolation, cultivation and differentiation of gram-negative enteric microorganisms isolated from both clinical and non-clinical specimens such as from feces, urine, and […]
X factor (hemin) and V factor (nicotinamide-adenine-dinucleotide, NAD) requirement test is done for the identification and differentiation of Haemophilus spp. especially identification of Haemophilus influenzae, which causes deadly diseases like meningitis, pneumonia among others. Haemophilus spp […]
The simple stain can be used as a quick and easy way to determine cell shape, size and arrangements of bacteria. True to its name, the simple stain is a very simple staining procedure involving […]
M. tuberculosis requires aerobic condition and a protein enriched medium for culture. Löwenstein–Jensen (LJ) slopes are main solid media used to culture Mycobacteria. It contains inspissated eggs, malachite green and glycerol (or pyruvate). LJ medium […]
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