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HomeBacteriologyAnaerobic Bacteriology

Anaerobic Bacteriology

Common features of Clostridium perfringens
Anaerobic Bacteriology

Clostridium perfringens: Properties, Diseases and Diagnosis

May 3, 2020 Acharya Tankeshwar 0

Clostridium perfringens is a gram-positive, anaerobic, endospore-forming bacilli, often appear as boxcar shaped. It is mainly responsible for myonecrosis (gas gangrene), food poisoning, and gangrenous cholecystitis. Properties Gram staining: Gram-variable straight rods with blunt ends […]

Clostridium tetani cover image
Anaerobic Bacteriology

Clostridium tetani: Properties, Pathogenesis and Laboratory diagnosis

May 2, 2020 Acharya Tankeshwar 0

Clostridium tetani is an obligate anaerobic, gram-positive bacillus with terminal round spore (drum stick appearance). It is the causative agent of tetanus, a vaccine-preventable disease that classically follows a puncture wound by a rusty nail […]

Clostridium botulinum cover image
Anaerobic Bacteriology

Clostridium botulinum: Properties, Pathogenesis and Laboratory Diagnosis

May 1, 2020 Acharya Tankeshwar 0

Clostridium botulinum is gram-positive, endospore-forming bacilli which resemble a tennis racket appearance due to the presence of spores at the sub-terminal end. It produces an extremely lethal neurotoxin called botulinum toxin and causes botulism, a […]

Toxins of Clostridium perfringens
Anaerobic Bacteriology

Toxins of Clostridium perfringens and their roles

April 6, 2020 Acharya Tankeshwar 0

Clostridium perfringens is a Gram-positive, anaerobic, spore-forming rod. It produces at least 12 different toxins, which are broadly classified as “major toxins” and “minor toxins”. Certain strains also produce enterotoxin and neuraminidase. These toxins are responsible for […]

anaerobic infections
Anaerobic Bacteriology

Anaerobic infections: Etiology, Characteristics and Diagnosis

February 29, 2020 Acharya Tankeshwar 2

Anaerobic infections range from local abscesses to life-threatening infections. In most of the cases, our own normal flora (i.e. endogenous origin) are involved but in some exogenous sources play a role. Anaerobic infections are normally […]

Anaerobic Bacteriology

Robertson’s Cooked Meat (RCM) Medium: Principle, Composition, Procedure and Uses

November 29, 2016 Acharya Tankeshwar 2

Robertson’s Cooked Meat (RCM) medium is used for the cultivation of aerobic, microaerophilic, and anaerobic microorganisms, especially Clostridium species.   It is also known as Cooked Meat Broth (CMB) as it contains pieces of fat-free minced […]

Anaerobic Bacteriology

Microbes with Characteristics Smell (good and bad)

October 17, 2016 Acharya Tankeshwar 4

Microorganisms can produce different types of volatile compounds that may give characteristics smell, pleasant scent, or pungent odor. Production of these volatile chemicals depends on the metabolic characteristics of that particular organism. Sometimes scent or […]

Anaerobic Bacteriology

Thioglycollate broth: Principle, Composition, Preparation and Uses

October 6, 2016 Nisha Rijal 8

Thioglycollate broth is the enrichment broth most frequently used in diagnostic bacteriology. This broth supports growth of anaerobes, aerobes, microaerophilic, and fastidious microorganisms.  It contains many nutritive factors, including casein, yeast, beef extracts, and vitamins […]

Anaerobic Bacteriology

McIntosh and Fildes’ anaerobic Jar: Principle, Procedure and Uses

August 19, 2016 Acharya Tankeshwar 3

McIntosh and Fildes’ anaerobic jar is an instrument used in Microbiology laboratory, for the generation of anaerobic condition (anaerobiosis) to culture obligates anaerobes such as Clostridium spp. Anaerobiosis obtained by McIntosh and Fildes’ anaerobic jar […]

Anaerobic Bacteriology

Clostridioides difficile: Characteristics, Disease and Laboratory Diagnosis

November 30, 2015 Acharya Tankeshwar 0

Clostridioides (formerly Clostridium) difficile causes life-threatening diarrhea. Clostridioides difficile infection (CDI) is a leading cause of hospital associated gastrointestinal illness.  This infections mostly occur in people who have had  recent medical care (hospitalized or recently hospitalized […]

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Anaerobic Bacteriology

Clinical Specimens suitable for anaerobic culture

May 26, 2013 Acharya Tankeshwar 0

Specimens for anaerobic culture should be properly collected and transported.  Indigenous anaerobes are often present in large numbers as normal flora on mucosal surfaces (e.g. mouth). So the sample from sites known to have anaerobes […]

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Anaerobic Bacteriology

Importance of Gram Stain in Anaerobic Bacteriology

May 26, 2013 Acharya Tankeshwar 4

Gram stain is an important rapid tool for anaerobic bacteriology. It reveals the types and relative numbers of microorganisms and host cells present. Gram stain also serves as a quality control measure for the adequacy […]

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