Clostridium perfringens is a gram-positive, anaerobic, endospore-forming bacilli, often appear as boxcar shaped. It is mainly responsible for myonecrosis (gas gangrene), food poisoning, and gangrenous cholecystitis. Properties Gram staining: Gram-variable straight rods with blunt ends […]
Clostridium tetani is an obligate anaerobic, gram-positive bacillus with terminal round spore (drum stick appearance). It is the causative agent of tetanus, a vaccine-preventable disease that classically follows a puncture wound by a rusty nail […]
Clostridium botulinum is gram-positive, endospore-forming bacilli which resemble a tennis racket appearance due to the presence of spores at the sub-terminal end. It produces an extremely lethal neurotoxin called botulinum toxin and causes botulism, a […]
Clostridium perfringens is a Gram-positive, anaerobic, spore-forming rod. It produces at least 12 different toxins, which are broadly classified as “major toxins” and “minor toxins”. Certain strains also produce enterotoxin and neuraminidase. These toxins are responsible for […]
Anaerobic infections range from local abscesses to life-threatening infections. In most of the cases, our own normal flora (i.e. endogenous origin) are involved but in some exogenous sources play a role. Anaerobic infections are normally […]
Robertson’s Cooked Meat (RCM) medium is used for the cultivation of aerobic, microaerophilic, and anaerobic microorganisms, especially Clostridium species. It is also known as Cooked Meat Broth (CMB) as it contains pieces of fat-free minced […]
Microorganisms can produce different types of volatile compounds that may give characteristics smell, pleasant scent, or pungent odor. Production of these volatile chemicals depends on the metabolic characteristics of that particular organism. Sometimes scent or […]
Thioglycollate broth is the enrichment broth most frequently used in diagnostic bacteriology. This broth supports growth of anaerobes, aerobes, microaerophilic, and fastidious microorganisms. It contains many nutritive factors, including casein, yeast, beef extracts, and vitamins […]
McIntosh and Fildes’ anaerobic jar is an instrument used in Microbiology laboratory, for the generation of anaerobic condition (anaerobiosis) to culture obligates anaerobes such as Clostridium spp. Anaerobiosis obtained by McIntosh and Fildes’ anaerobic jar […]
Clostridioides (formerly Clostridium) difficile causes life-threatening diarrhea. Clostridioides difficile infection (CDI) is a leading cause of hospital associated gastrointestinal illness. This infections mostly occur in people who have had recent medical care (hospitalized or recently hospitalized […]
Specimens for anaerobic culture should be properly collected and transported. Indigenous anaerobes are often present in large numbers as normal flora on mucosal surfaces (e.g. mouth). So the sample from sites known to have anaerobes […]
Gram stain is an important rapid tool for anaerobic bacteriology. It reveals the types and relative numbers of microorganisms and host cells present. Gram stain also serves as a quality control measure for the adequacy […]
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