Anaerobic bacteria are both friend and foe for us. As a normal microbiota of exposed body surfaces and mucous membranes, they help us by digesting foodstuffs, preventing attachment of pathogens, and supplying vitamins such as vitamin K. But when they are introduced to a new site in the body they can cause infections such as liver abscess, brain abscess, lungs abscess, myonecrosis, gas gangrene, and oral anaerobic infections.
To recover anaerobic bacteria from clinical specimens appropriate collection, transport, and processing procedures must be followed.
Bacteroides fragilis is a gram-negative, pleomorphic anaerobe responsible for variety of anaerobic infections including peritonitis.
Commensal in the large intestine of humans and is responsible for the disease, gas gangrene.
Gram positive, obligate anaerobe, looks like drum-stick shaped and causes tetanus.
Gram positive, anaerobic rod which produces powerful neurotoxin "botulinum toxin".
Alpha (α) toxin, Beta (β) toxin, Epsilon (ε) toxin, and Iota (ι) toxin are the major toxins of Clostridium perfringens.
Anaerobic bacteria are part of the normal flora but can cause abscess formation and tissue necrosis when enter into sterile sites.
The most widely used anaerobic culture medium containing chopped meat particles and used for maintenance of stock cultures.
Odor of colonies of bacteria may help in their identification but sniffing culture plates is not a good idea and you may contract a disease.
Thioglycollate broth supports the growth of anaerobes, aerobes, microaerophilic, and fastidious microorganisms.
McIntosh and Fildes' anaerobic jar works on the principle of evacuation and replacement, where the air inside the chamber is evacuated and replaced with mixture of gases
Gram positive obligate anaerobe responsible for a unique colonic disease, pseudomembranous colitis.
Sample from sites known to have anaerobes as part of the normal flora (eg mouth) is unacceptable for anaerobic culture.
Positive gram stain with negative culture report gives information regarding the adequacy of sample collection, transport, and also of culture methods used.