Microorganisms can produce different types of volatile compounds which may give characteristics smell, pleasant scent or pungent odor. Production of these volatile chemicals depends on the metabolic characteristics of that particular organism. Sometimes scent or […]
Thioglycollate broth is the enrichment broth most frequently used in diagnostic bacteriology. This broth supports growth of anaerobes, aerobes, microaerophilic, and fastidious microorganisms. It contains many nutrient factors, including casein, yeast, and beef extracts, and […]
McIntosh and Fildes’ anaerobic jar is an instrument used in Microbiology laboratory, for the generation of anaerobic condition (anaerobiosis) to culture obligates anaerobes such as Clostridium spp. Anaerobiosis obtained by McIntosh and Fildes’ anaerobic jar […]
Clostridium difficile (C. difficile) causes life-threatening diarrhea. Clostridium difficile infection (CDI) is a leading cause of hospital associated gastrointestinal illness. This infections mostly occur in people who have had recent medical care (hospitalized or recently hospitalized […]
Specimens for anaerobic culture should be properly collected and transported. Indigenous anaerobes are often present in large numbers as normal flora on mucosal surfaces (e.g. vagina, mouth). So the sample from sites known to have […]
Gram stain is an important rapid tool for anaerobic bacteriology. It reveals the types and relative numbers of microorganisms and host cells present. Gram stain also serves as a quality control measure for the adequacy […]
Media used in the anaerobic bacteriology can be freshly prepared or purchased from commercial suppliers. Media for anaerobic culture prepared in the laboratory should be used within 2 weeks of preparation as long storage degrades the quality of […]
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