Anaerobic Bacteriology

Thioglycollate broth

Last updated on June 21st, 2021Thioglycollate broth is the enrichment broth most frequently used in diagnostic bacteriology. This broth supports the growth of anaerobes, aerobes, microaerophilic, and fastidious microorganisms.  It contains many nutritive factors, including […]

Eukaryotic and Prokaryotic cells
Bacteriology

Prokaryotic Cell vs. Eukaryotic Cell

Last updated on June 23rd, 2021All living cells can be classified as either prokaryotic or eukaryotic. Bacteria are prokaryotic, while fungi, protozoa, and other organisms are eukaryotic. Eukaryotic cells contain a nucleus with a nuclear […]

Nagler's test
Bacteriology

Nagler Reaction (Lecithinase Test)

Last updated on June 21st, 2021Lecithinase test or Nagler’s reaction is a biochemical test used to identify organisms that liberate phospholipases (lecithinases) e.g. Clostridium perfringens. The alpha (α) toxin of C. perfringens has phospholipase activity […]

Albert Stain
Bacteriology

Albert Stain: Principle, Procedure and Results

Last updated on May 22nd, 2021Albert stain is a type of differential stain used for staining high-molecular-weight polymers of polyphosphate known as metachromatic granules or volutin granules found in Corynebacterium diphtheriae. Metachromatic granules are also […]

Culture Media

Baird Parker Agar: Preparation, Uses

Last updated on June 17th, 2021Baird Parker Agar was developed by Baird Parker in 1962 and is a modification of the Tellurite-glycine formulation of Zebovitz et al. It is a moderately selective medium for the […]

Culture Media

Xylose Lysine Deoxycholate (XLD) Agar

Last updated on June 24th, 2021Xylose lysine deoxycholate (XLD) agar is both a selective and differential medium for the isolation, cultivation and differentiation of gram-negative enteric microorganisms. This media is primarily used for isolation and […]

Viral Transport Medium (VTM)
Culture Media

Transport Media Used in Microbiology Lab

Last updated on June 21st, 2021Transport media are essentially solutions of buffers with carbohydrates, peptones and other nutrients (excluding growth factors) designed to preserve the viability of bacteria during transport without allowing their multiplication. The […]