Last updated on June 21st, 2021Thioglycollate broth is the enrichment broth most frequently used in diagnostic bacteriology. This broth supports the growth of anaerobes, aerobes, microaerophilic, and fastidious microorganisms. It contains many nutritive factors, including […]
Last updated on June 23rd, 2021All living cells can be classified as either prokaryotic or eukaryotic. Bacteria are prokaryotic, while fungi, protozoa, and other organisms are eukaryotic. Eukaryotic cells contain a nucleus with a nuclear […]
Last updated on June 21st, 2021Lecithinase test or Nagler’s reaction is a biochemical test used to identify organisms that liberate phospholipases (lecithinases) e.g. Clostridium perfringens. The alpha (α) toxin of C. perfringens has phospholipase activity […]
Last updated on June 2nd, 2021The Mantoux tuberculin skin test is the standard method of determining whether a person is infected with Mycobacterium tuberculosis or has exposure to tubercle bacilli. It was developed by Koch […]
Last updated on June 2nd, 2021Acridine orange is a dye that intercalates or binds with the nucleic acid ( either DNA or RNA) present in organisms and fluoresce to emit various colors that help in […]
Last updated on June 17th, 2021Sabouraud Agar or Sabouraud Dextrose Agar (SDA) is a selective medium primarily used for the isolation of dermatophytes. Other fungi, yeasts, and filamentous bacteria such as Nocardia can also grow […]
Last updated on May 22nd, 2021Albert stain is a type of differential stain used for staining high-molecular-weight polymers of polyphosphate known as metachromatic granules or volutin granules found in Corynebacterium diphtheriae. Metachromatic granules are also […]
Last updated on June 17th, 2021Potato dextrose agar (PDA) is a general-purpose basal medium for the identification, cultivation, and enumeration of yeast and molds in foods and dairy products. It may also be used for […]
Last updated on June 17th, 2021Baird Parker Agar was developed by Baird Parker in 1962 and is a modification of the Tellurite-glycine formulation of Zebovitz et al. It is a moderately selective medium for the […]
Last updated on June 21st, 2021Cetrimide agar also known as pseudosel agar is used for the selective isolation and presumptive identification of Pseudomonas aeruginosa. The medium was first developed by Lowburry and is a modification […]
Last updated on June 24th, 2021Xylose lysine deoxycholate (XLD) agar is both a selective and differential medium for the isolation, cultivation and differentiation of gram-negative enteric microorganisms. This media is primarily used for isolation and […]
Last updated on June 21st, 2021Transport media are essentially solutions of buffers with carbohydrates, peptones and other nutrients (excluding growth factors) designed to preserve the viability of bacteria during transport without allowing their multiplication. The […]
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