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Home2015February

Month: February 2015

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Bacteriology

Tween 80 hydrolysis test: Principle, Procedure and results

February 17, 2015 Acharya Tankeshwar 1

Tween 80 is the trade name of the  detergent polyethylene derivative of sorbitan mono-oleate. Some Mycobacterium species posses an enzyme-lipase, that splits the compound and releases oleic acid  and polyoxyethylated sorbitol. Positive reactions occur in […]

Lab Diagnosis of Viral Disease

Japanese Encephalitis (JE) Virus: Structure, life cycle, pathogenesis and diagnosis

February 15, 2015 Acharya Tankeshwar 0

Japanese Encephalitis (JE) virus was initially isolated in Japan in 1935. It is a leading cause of vaccine-preventable encephalitis (inflammation of the brain) in Asia and the western Pacific. The virus is maintained in an […]

Antibiotic Resistance

Teixobactin, a novel antibiotic: Introduction, Mechanism of Action and Uses

February 9, 2015 Acharya Tankeshwar 2

A group of researcher published an article in “Nature” reporting the discovery of a new cell wall inhibitor, teixobactin, from a screen of uncultured bacteria grown in diffusion chambers in situ using “ iChip” technology. […]

Bacteriology

ONPG Test (for β-galactosidase): Principle, procedure and results

February 6, 2015 Acharya Tankeshwar 4

O-Nitrophenyl-β-D-galactopyranoside (ONPG) is structurally similar to lactose (i.e. ONPG is an analog of lactose), except that orthonitrophenyl has been substituted for glucose. On hydrolysis, through the action of the enzyme β-galactosidase, ONPG cleaves into two […]

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Bacteriology

Transport Medium used in Microbiology Laboratory

February 5, 2015 Nisha Rijal 4

Transport media are essentially solutions of buffers with carbohydrates, peptones and other nutrients (excluding growth factors) designed to preserve the viability of bacteria during transport without allowing their multiplication. The primary objective of the use […]

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Bacteriology

Leucine Amino peptidase (LAP) Test: Principle, Procedure and results

February 5, 2015 Acharya Tankeshwar 4

Leucine Amino peptidase (LAP) Test is used to identify catalase negative gram-positive cocci. The LAP disk is a rapid test for the detection of enzyme lucine amino peptidase. Leucine-beta-naphthylamide impregnated disk serve as a substrate […]

Bacteriology

Gram Staining: Principle, Procedure and Results

February 2, 2015 Acharya Tankeshwar 48

Gram staining method, the most important procedure in Microbiology, was developed by Danish physician Hans Christian Gram in 1884. Gram staining is still the cornerstone of bacterial identification and taxonomic division. This differential staining procedure separates […]

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