Tween 80 is the trade name of the detergent polyethylene derivative of sorbitan mono-oleate. Some Mycobacterium species posses an enzyme-lipase, that splits the compound and releases oleic acid and polyoxyethylated sorbitol. Positive reactions occur in […]
Japanese Encephalitis (JE) virus was initially isolated in Japan in 1935. It is a leading cause of vaccine-preventable encephalitis (inflammation of the brain) in Asia and the western Pacific. The virus is maintained in an […]
A group of researcher published an article in “Nature” reporting the discovery of a new cell wall inhibitor, teixobactin, from a screen of uncultured bacteria grown in diffusion chambers in situ using “ iChip” technology. […]
O-Nitrophenyl-β-D-galactopyranoside (ONPG) is structurally similar to lactose (i.e. ONPG is an analog of lactose), except that orthonitrophenyl has been substituted for glucose. On hydrolysis, through the action of the enzyme β-galactosidase, ONPG cleaves into two […]
Transport media are essentially solutions of buffers with carbohydrates, peptones and other nutrients (excluding growth factors) designed to preserve the viability of bacteria during transport without allowing their multiplication. The primary objective of the use […]
Leucine Amino peptidase (LAP) Test is used to identify catalase negative gram-positive cocci. The LAP disk is a rapid test for the detection of enzyme lucine amino peptidase. Leucine-beta-naphthylamide impregnated disk serve as a substrate […]
Gram staining method, the most important procedure in Microbiology, was developed by Danish physician Hans Christian Gram in 1884. Gram staining is still the cornerstone of bacterial identification and taxonomic division. This differential staining procedure separates […]
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