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Home2013May

Month: May 2013

Biochemical tests in Microbiology

Optochin Sensitivity Test: Principle, Procedure, Results and Quality Control

May 17, 2013 Acharya Tankeshwar 7

Streptococcus pneumoniae strains are sensitive to the chemical optochin (ethylhydrocupreine hydrochloride). Optochin sensitivity allows for the presumptive identification of alpha-hemolytic streptococci as S. pneumoniae, although some pneumococcal strains are optochin-resistant. Other alpha-hemolytic streptococcal species are […]

Biochemical tests in Microbiology

Bile Solubility test: Principle, Procedure, and Results

May 17, 2013 Acharya Tankeshwar 4

The bile (sodium deoxycholate) solubility test distinguishes Streptococcus pneumoniae from all other alpha-hemolytic streptococci.  Streptococcus pneumoniae is bile soluble whereas all other alpha-hemolytic streptococci are bile resistant. Sodium deoxycholate (2% in water) will lyse the […]

Bacteriology

Citrate Utilization Test: Principle, Procedure and Results

May 17, 2013 Acharya Tankeshwar 9

Citrate utilization test is commonly employed as part of a group of tests, the IMViC (Indole, Methyl Red, VP and Citrate)  tests, that distinguish between members of the Enterobacteriaceae family based on their metabolic by-products. […]

Bacteriology

Bacitracin Test: Principle, Procedure, expected results

May 17, 2013 Acharya Tankeshwar 6

Bacitracin is a bactericidal drug useful in the treatment of superficial skin infections but too toxic for systemic use. Bacitracin is a polypeptide antibiotic produced by Bacillus subtilis. This drug interferes in the peptidoglycan synthesis […]

Bacteriology

Streptococcus pneumoniae (pneumococcus): Disease, Properties, Pathogenesis and Laboratory diagnosis

May 13, 2013 Acharya Tankeshwar 2

Streptococcus pneumoniae is a Gram positive cocci, typically lancet shaped and occurs singly, in pairs (diplococci) or in short chains. Main diseases caused by Streptococcus pneumoniae: Bacterial pneumonia: Most common bacterial cause, especially in infants and […]

Bacteriology

Wolbachia Bacteria may make mosquitoes resistant to malaria parasite

May 11, 2013 Acharya Tankeshwar 0

Malaria is a parasitic disease which is transmitted by the bite of infected female Anopheles mosquito which is harboring (carrying) Plasmodium spp ( protozoan responsible for malaria).  In the latest study from Michigan State University […]

Bacteriology

Bacterial growth curve: phases and significance

May 11, 2013 Acharya Tankeshwar 7

This bacterial growth curve reflects the events in bacterial population when they are grown in a closed system of microbial culture of fixed volume (i.e. batch culture).The typical growth curve for population of cells can […]

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Microbiology for Beginners

Biosafety level 2 (BSL2) guidelines for teaching laboratories

May 9, 2013 Acharya Tankeshwar 0

Preamble: Educators need to be aware of the risks inherent in using microorganisms in the laboratory and must use best practices to minimize the risk to themselves, students, and the community. The following guidelines are […]

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Bacteriology

MCQs on Sexually Transmitted Infections and spiral bacteria

May 9, 2013 Acharya Tankeshwar 2

MCQ Bacteriology 59: A 70 year old woman with severe chronic lung disease presented in the OPD of Tribhuvan University Teaching Hospital with fever, productive cough with purulent sputum and worsening hypoxemia. Sputum sample was […]

Bacteriology

Oxygen Requirements for Pathogenic Bacteria

May 9, 2013 Acharya Tankeshwar 11

Oxygen requirements of bacteria reflect the mechanism used by them, to satisfy their energy needs. On the basis of oxygen requirements, bacteria can be divided into following different categories: Aerobes: Aerobes grow in ambient air, which contains 21% […]

No Picture
Bacteriology

Biosafety level 1 (BSL1) guidelines for teaching laboratories

May 8, 2013 Acharya Tankeshwar 2

Preamble: Educators need to be aware of the risks inherent in using microorganisms in the laboratory and must use best practices to minimize the risk to students and the community. The following guidelines are designed […]

Overview of Biochemical tests for differentiating Gram positive cocci
Biochemical tests in Microbiology

Overview of Biochemical tests used to identify bacteria in Microbiology laboratory

May 7, 2013 Acharya Tankeshwar 36

In this blog post, i am sharing information about common, conventional biochemical tests and their primary uses in Microbiology laboratory for the identification of particular pathogen of interest. Some of the biochemical tests mentioned here […]

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