A. Aerobic Bacteria Main Principle: Provide Oxygen Atmospheric condition is generally satisfactory for culture of aerobes or facultative anaerobes but for the growth of many aerobes, it is necessary to provide extensive aeration. Forced aeration […]
Membrane filters have a known uniform porosity of predetermined size (generally 0.45 µm ) sufficiently small to trap microorganisms.Using the membrane filter technique, sample is passed through the membrane using a filter funnel and vacuum system. Any […]
Once a microorganism has been isolated and grown in pure culture, it becomes necessary to maintain the viability and purity of the microorganism by keeping the pure culture free from contamination. Normally in laboratories, the […]
Enumeration of microorganisms is especially important in dairy microbiology, food microbiology, and water microbiology. Direct Microscopic count/ Total cell count Direct microscopic counts are possible using special slides known as counting chambers, consisting of […]
Culture media contains nutrients and physical growth parameters necessary for microbial growth. All microorganisms cannot grow in a single culture medium and in fact many can’t grow in any known culture medium. Organisms that cannot […]
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